Enzyme engineering

Under the trend of the global chemical process to the green  not polluted，green chemical synthesis route has become more and more attention. When products will be put into industrial production, chemical enterprise how to realize zero discharge of pollutants in production processes, while maintaining or even lower cost and industrial products of high yield this problem become the focus of many chemical production enterprises. BSAZ relying on the international advanced synthetic technology and its strong scientific research team, in green synthesis of thoughts as the instruction, will be introduced to the enzymatic synthesis, and then has brought new opportunities in the field of organic synthesis. The specificity and selectivity of enzymatic synthesis have obvious advantages compared with chemical synthesis, and the use of microbial and enzyme regions, sites, and three-dimensional selectivity, for example hydroxylation, epoxidation, isomerization, hydrolyze, enantiomeric resolution, synthesis of pharmaceutical intermediates, some of these reactions are difficult to achieve by chemical methods. The directional adjustment and control in enzymatic can multiple increases the efficiency of biotransformation and biosynthesis rate, and change the direction of reaction equilibrium. Enzymatic synthesis of biological functional molecules, non-natural useful material and functional polymer materials, applied to the production of chemical materials, the electronics industry has become possible. Enzyme plays an important role which is asymmetric synthesis or split chiral drug including alcohol, aldehyde, ketone, acid, amine, amide, amino acids, antibiotics, glycosidase inhibitors and antiviral drugsin organic synthesis, such as pesticide, medicine, spices, pesticides, in addition to pesticides, insects, hormone, pheromones, etc. Only the specific structure of chiral has bioactivity, it directly related to the pharmacological effects, side effects, efficacy and efficacy, etc. In organic materials, such as liquid crystal, target compound, semiconductor and conductive functional polymer materials, the chiral component determines its physical properties.
      Hydrolytic enzymes, REDOX enzymes, cracking enzymes, connecting synthetic enzymes, heterogeneous enzymes, and transferases, all of these enzymes can be used in the synthesis of organic compounds and chiral compounds. For example lipase can be widely used in the synthesis of a variety of amino acids, carboxylic acids, chiral alcohols and so on. The enzyme in non-aqueous phase esterification or transesterification can be splatted to racemic carboxylic acid by optical voidness and only chiral drug intermediates. Protease is kitting for irreversible polypeptide chain synthesis. Glycosyl transferase can synthesize glycosylated proteins with medical value. Alcohol dehydrogenase and hydroxide radical alcohol dehydrogenase can catalysis hydroxyl – ketoneredox reactions, preparation of drugs, pheromone, steroids, three carboxylic acid chrome complex and synthetic fiber, etc. Alcohol dehydrogenase of yeast is mainly catalyzed fatty alcohol and aldehyde ketoneby redox reactions. The horse liver alcohol dehydrogenase (HLADH) cans specific redox fat cyclic alkanol or aldehyde ketone, however, steroid Dehydrogenaseis mainly catalyzed condensed nucleus fatty alcohol and aldehyde ketone by redox reactions. Oxidase can synthesis chain alkene compound, cyclase can synthesis steroids and terpene compound.
      Enzymes can be synthesized featured functional polymer materials, for example peroxidase (POD) can catalyzed the polymerization of phenol and aromatic amine, this kind of molecular materials rigidity enhancement, conductivity apparentness, complexation with metal ions, electroluminescence, and can preparation for nano materials. Tyrosinase polymerization dopamine cans synthesis polypyrrole (PPY) with conductivity, which is used for biological sensors. Lipase and alkaline protease catalyze hydroxy carboxylic acid ester condensation in non-aqueous medium turn into polyester polymer or chitosan. Macrolide generally is used for synthesis antibiotic biosynthetic intermediates, additives spice, insect sex pheromone, plant growth regulator and liquid crystal compounds intermediates, polyester can be biodegradable, using for the control of drug release, packaging materials to eliminate white pollution
      There are some obvious differences compared with chemical method on the structure, properties and functions, so enzymatic polymers have the advantage of chemical methods cannot have. Chiral molecule biosynthesis is still in the exploratory stage, according to improve the selectivity of enzyme catalysis and to optimize the synthesis process, it can be predicted that someone can make important progress in the near future.